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*In vitro* Kinase Assays {#s4h}
————————

The kinase assay was performed using 50 ng of recombinant human DYRK1A protein (Upstate Biotechnology) and 250 nM of the exogenous receptor protein in 50 µl reaction buffer (50 mM Tris-HCl, pH 7.5, 10 mM MnCl~2~, 5 mM MgCl~2~, 0.1 mg/ml BSA) supplemented with 1 mM dithiothreitol, 20 mM NaCl, 1 mM NaVO~4~, and 0.1 mM PMSF at 30°C. The phosphorylation reaction was initiated by the addition of [γ-^32^P]ATP (1,000 Ci/mmol) to the mixture and stopped by addition of 100 µl of 5× SDS-PAGE sample buffer containing 100 mM EDTA. The samples were subjected to SDS-PAGE followed by autoradiography and phosphorimaging analysis.

Thymocyte Cell Sorting {#s4i}
———————-

E11 and Flk1^+^ thymocytes were sorted using a BD FACSAria II (Becton-Dickinson) cell sorter.

Generation of Lineage Reporter Mice {#s4j}
———————————–

*Sox7^Cre/+^;Dyrk1a^flox/flox^* mice were crossed with *R26R^tdTomato/tdTomato^* mice [@pbio.1000129-Srinivas1] to generate *Sox7^Cre/+^;Dyrk1a^flox/flox^;R26R^tdTomato/tdTomato^* triple transgenic mice.

Supporting Information {#s5}
======================
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